Proliferation kinetics and mineralization properties of MC3T3‐E1 cells on whey protein isolate scaffolds for bone tissue regeneration

The purpose of the study is to assess influence scaffold composition and properties on cellular proliferation mineralization a whey protein isolate (WPI) scaffold. Scaffolds varying concentrations WPI, calcium chloride, amylopectin are seeded with cells, growth curves constructed from cell density measurements following incubation. Non-linear regression yields kinetic parameters which compared for quantitative assessment performance. show biocompatibility ability sustain culture length study. For up 14 days, data well described by exponential growth. Thereafter, saturation behavior appears, fit sigmoidal model. seeding efficiency improves increasing WPI concentration, peaking at 5 mM CaCl2, decreases added amylopectin. constant almost independent composition, possible increases CaCl2 retardation composition. cultured in differentiation conditions, shows development mineralized matrix both acellular surfaces osteoblast cultures. unmodified gel promote model preosteoblast provides valuable trends concentration effects further design.